| Organism Summary | |
|---|---|
| Catalog Number | ATCC® 14474™ | 
| Strain Alias | D-25 [TMC 1541] | 
| Isolation | Guinea pigs with tuberculosis arrested by chemotherapy | 
| Tags | Methylation DataType Strain | 
| Biosafety Level | 2 | 
| Type Strain | Yes | 
| Assembly Summary | |
|---|---|
| Date Published | October 3, 2023 | 
| Length | 5,987,972 nt | 
| Sequencing Technology | Illumina + Oxford Nanopore Hybrid Assembly | 
| Number of Contigs | 2 (All Circularized) | 
| N50 | 5,958,939 nt | 
| %GC | 68.48% | 
| Annotations Summary | |
|---|---|
| Number of CDS | 5793 | 
| Number of Hypothetical Proteins | 700 | 
| Number of tRNA | 47 | 
| Number of 5s rRNA | 2 | 
| Number of 16s rRNA | 2 | 
| Number of 23s rRNA | 2 | 
| Input Reads Summary | |
|---|---|
| Oxford Nanopore Read Count | 223,207 | 
| Oxford Nanopore Median Q Score | 12.9 | 
| Illumina Read Count | 10,460,938 | 
| Illumina Mean Coverage Depth | 255.411× | 
| Illumina Median Q Score | 34 | 
| Contig | Start | End | Name | Protein Product | EC Number | Type | RefSeq ID | Jump | 
|---|
The following genomes have the greatest genomic similarity to this one (≥95% average nucleotide identity):
| Taxonomic Name | ATCC Product Page | Strain Alias | Tags | Date Published | Length | Download | 
|---|
| Sequencing Quality Control | |
|---|---|
| Number of trimmed reads | 10,460,938 | 
| Median Q score, all bases | 34 | 
| Percent of median Q scores per cycle greater than 25 | 1% | 
| Ambiguous content (% N bases) | 0% | 
| Assembly Quality Control | |
|---|---|
| Estimated genome completeness | 100% | 
| Estimated genome contamination | 0% | 
| Illumina depth of coverage | 255.411× | 
| ONT depth of coverage | 322.528× | 
This bacterium was assembled, annotated, and curated using Oatmeal v1.2. See our technical documentation for further details: https://docs.onecodex.com/en/articles/5357065-assembly-qc-and-annotation-pipeline-versions