ATCC® 20™ | Mycobacterium smegmatis

Overview

Genome Browser

Related Genomes

Quality Control

Assembly Summary
Date Published	March 20, 2026
Length	7,074,932 nt
Sequencing Technology	Illumina + Oxford Nanopore Hybrid Assembly
Number of Contigs	1 (All Circularized)
N50	7,074,932 nt
%GC	67.36%

Annotations Summary
Number of CDS	6731
Number of Hypothetical Proteins	674
Number of tRNA	47
Number of 5s rRNA	2
Number of 16s rRNA	2
Number of 23s rRNA	2

Input Reads Summary
Oxford Nanopore Read Count	390,876
Oxford Nanopore Median Q Score	46
Illumina Read Count	6,462,562
Illumina Mean Coverage Depth	134.115×
Illumina Median Q Score	40

The following genomes have the greatest genomic similarity to this one (≥95% average nucleotide identity):

All Microbes

0 out of 0 selected (Select All / Deselect All).

		Taxonomic Name	ATCC Product Page	Strain Alias	Tags	Date Published	Length

Displaying Product

Sequencing Quality Control

Sequencing Quality Control Metrics
Number of trimmed reads	6,462,562
Median Q score, all bases	40
Percent of median Q scores per cycle greater than 25	1%
Ambiguous content (% N bases)	0%

Assembly Quality Control Metrics
Estimated genome completeness	97.26%
Estimated genome contamination	3.53%
Illumina depth of coverage	134.115×
ONT depth of coverage	221.876×

This bacterium was assembled, annotated, and curated using Oatmeal v3.1. See our technical documentation for further details: https://docs.onecodex.com/en/articles/5357065-assembly-qc-and-annotation-pipeline-versions